TCP1-eta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88144

Novus Biologicals, part of Bio-Techne
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NBP1-88144
NBP1-88144-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TCP1-eta Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ELKAEKDNAEIRVHTVEDYQAIVDAEWNILYDKLEKIHHSGAKVVLSKLPIGDVATQYFADRDMFCAGRVPEEDLKRTMMACGGSIQTSVNALSADVLGRCQVFEETQIGGERYNFFTGCPKAKTCTFILRGGAEQFMEETE

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24213632)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCP1-eta Antibody

Western Blot: TCP1-eta Antibody [NBP1-88144]

Western Blot: TCP1-eta Antibody [NBP1-88144]

Western Blot: TCP1-eta Antibody [NBP1-88144] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CCT7 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: TCP1-eta Antibody [NBP1-88144]

Immunocytochemistry/ Immunofluorescence: TCP1-eta Antibody [NBP1-88144]

Immunocytochemistry/Immunofluorescence: TCP1-eta Antibody [NBP1-88144] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TCP1-eta Antibody [NBP1-88144]

Immunohistochemistry-Paraffin: TCP1-eta Antibody [NBP1-88144]

Immunohistochemistry-Paraffin: TCP1-eta Antibody [NBP1-88144] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
View 1 more images

Applications for TCP1-eta Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCP1-eta

TCP1-eta encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants encoding different isoforms have been found for this gene, but only two of them have been characterized to date.

Alternate Names

CCTETA, CCT-eta, CCTH, chaperonin containing TCP1, subunit 7 (eta), eta subunit, HIV-1 Nef interacting protein, HIV-1 Nef-interacting protein, MGC110985, Nip7-1, T-complex protein 1 subunit eta

Gene Symbol

CCT7

Additional TCP1-eta Products

Product Documents for TCP1-eta Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TCP1-eta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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