TCR alpha/beta Antibody (Desire-1) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09021
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # Desire-1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
mAb desire-1 was obtained after fusion of spleen cells from a BALB.K mouse immunised twice with KB5-C20 clone cells in RPMI 1640 medium, with non-Ig-producing myeloma cells X63-Ag8.653. Desire-1 was selected for its capacity to inhibit selectively the H-2Kb-specific cytolysis by clone KB5-C20.
Specificity
mAb desire-1 recognises an epitope contributed by both the alpha and beta chains of the KB5-C20 TCR.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for TCR alpha/beta Antibody (Desire-1) - Chimeric - Azide and BSA Free
![Immunocytochemistry/ Immunofluorescence: TCR alpha/beta Antibody (Desire-1) - Chimeric - Azide and BSA Free [NBP3-09021]](https://resources.bio-techne.com/images/products/TCR-alpha-beta-Antibody-Desire-1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09021-img0001.jpg "Immunocytochemistry/ Immunofluorescence: TCR alpha/beta Antibody (Desire-1) - Chimeric - Azide and BSA Free [NBP3-09021]")
Immunocytochemistry/ Immunofluorescence: TCR alpha/beta Antibody (Desire-1) - Chimeric - Azide and BSA Free [NBP3-09021]
Immunocytochemistry/Immunofluorescence: TCR alpha/beta Antibody (Desire-1) - Chimeric [NBP3-09021] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of Desire-1 (NBP3-09021) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09021, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.Applications for TCR alpha/beta Antibody (Desire-1) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Desire-1 has been used for the immunoprecipitation of soluble TCR molecules (Gregoire 1991 & Hua 1986). An FITC conjugated desire-1 has been used in flow cytometric analysis as a marker for clonotype expression (Schonrich 1991). Further, a Fab fragment derived from desire-1 has been used in the crystallisation of the T-cell receptor for 3D structure determination (Housset 1997).
Desire-1 has been used for the immunoprecipitation of soluble TCR molecules (Gregoire 1991 & Hua 1986). An FITC conjugated desire-1 has been used in flow cytometric analysis as a marker for clonotype expression (Schonrich 1991). Further, a Fab fragment derived from desire-1 has been used in the crystallisation of the T-cell receptor for 3D structure determination (Housset 1997).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TCR alpha/beta
Alternate Names
C), D, FLJ22602, J, MGC117436, MGC22624, MGC23964, MGC71411, T cell receptor alpha locus, T-cell antigen receptor, alpha polypeptide, T-cell receptor, alpha (V, TCRA, TCRD, TRA
Gene Symbol
TRA
Additional TCR alpha/beta Products
Product Documents for TCR alpha/beta Antibody (Desire-1) - Chimeric - Azide and BSA Free
Product Specific Notices for TCR alpha/beta Antibody (Desire-1) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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