TCR alpha/beta Antibody (R73) - Low Endotoxin and Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05296

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Rat, Non-human Primate

Applications

Flow Cytometry, Functional, Immunocytochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse Clone # R73

Format

Low Endotoxin and Azide Free

Concentration

1 mg/ml

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Product Summary for TCR alpha/beta Antibody (R73) - Low Endotoxin and Azide Free

Immunogen

Rat T blasts and erythrocytes

Specificity

The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.

Clonality

Monoclonal

Host

Mouse

Scientific Data Images for TCR alpha/beta Antibody (R73) - Low Endotoxin and Azide Free

TCR alpha/beta Antibody (R73) - Low Endotoxin and Azide Free Flow Cytometry: TCR alpha/beta Antibody (R73) - Low Endotoxin and Azide Free [NBP3-05296]

Flow Cytometry: TCR alpha/beta Antibody (R73) - Low Endotoxin and Azide Free [NBP3-05296]

Separation of rat TCR alpha/beta positive thymocytes (red-filled) from TCR alpha/beta negative thymocytes (black-dashed) in Analysis (surface staining) of rat thymocyte suspension stained using anti-rat TCR alpha/beta (R73) purified antibody (concentration in sample 1.6 ug/ml) GAM APC.

Applications for TCR alpha/beta Antibody (R73) - Low Endotoxin and Azide Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml
Application Notes
Functional application: In vitro activation and in vivo depletion of alpha/beta TCR T cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Format

Low Endotoxin and Azide Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TCR alpha/beta

MAb TCR2 precipitates a CD3-associated heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50-kDa upon reduction) on chicken peripheral blood T cells. (1) Deglycosylation of the heterodimer yields two polypeptides of Mr 34-kDa and 29-kDa from TCR2 precipitates. In the chicken, two distinct subpopulations of alpha beta T cells appear in the thymus subsequent to the appearance of gamma delta T cells. These subpopulations, originally denoted as TCR2 and TCR3, (1-3) arise sequentially in the thymus during ontogeny and are now known to represent two distinct V beta families, Vbeta1 and Vbeta2, respectively. (4) The TCR2 MAb reacts with approximately 40% of thymocytes, 40-50% of blood mononuclear cells and 40% of splenocytes in the chicken. Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74+-2%) or CD8 (26+-4%). TCR2+ cells in the spleen also express either CD4 (37+-1%) or CD8 (64+-4%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2. (1-3) This observation led to the demonstration that 71+-6% of the TCR1(gamma delta)+ cells in the spleen express the CD8 marker. (1-4)

Alternate Names

C), D, FLJ22602, J, MGC117436, MGC22624, MGC23964, MGC71411, T cell receptor alpha locus, T-cell antigen receptor, alpha polypeptide, T-cell receptor, alpha (V, TCRA, TCRD, TRA

Gene Symbol

TRA

Additional TCR alpha/beta Products

Product Documents for TCR alpha/beta Antibody (R73) - Low Endotoxin and Azide Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TCR alpha/beta Antibody (R73) - Low Endotoxin and Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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