TDG Antibody (622)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43717
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (91%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 622
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary.
Reactivity Notes
Mouse (88%), Rat (84%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TDG Antibody (622)
![Western Blot: TDG Antibody (622) [NBP2-43717]](https://resources.bio-techne.com/images/products/TDG-Antibody-622-Western-Blot-NBP2-43717-img0002.jpg "Western Blot: TDG Antibody (622) [NBP2-43717]")
Western Blot: TDG Antibody (622) [NBP2-43717]
Western Blot: TDG Antibody (622) [NBP2-43717] - RNAseq analysis showed that essential factors of the BER pathway were statistically significantly down-regulated (p<0.05) in 96-h induced Saos2- (i) and Li-Fraumeni- (ii) p21WAF1/Cip1 Tet-ON cells (see also Additional file 1: Figure S3 for specific real time RT-PCR validation). Note that although in Saos2- p21WAF1/Cip1 Tet-ON cells OGG1 expression was not found by RNAseq analysis, specific real-time RT-PCR and microarray analysis (see also Additional file 1: Figure S3) [9] confirmed its decreased expression. Selective immunoblots for APEX1, LIG3, TDG, and MUTY confirmed the specificity of the RNA analysis results. a-Tubulin served as loading control. Image collected and cropped by CiteAb from the following publication (genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1401-9), licensed under a CC-BY license.![Western Blot: TDG Antibody (622) [NBP2-43717]](https://resources.bio-techne.com/images/products/TDG-Antibody-622-Western-Blot-NBP2-43717-img0001.jpg "Western Blot: TDG Antibody (622) [NBP2-43717]")
Western Blot: TDG Antibody (622) [NBP2-43717]
Western Blot: TDG Antibody (622) [NBP2-43717] - Analysis of 293T mock transfected (-) or transfected with a construct expressing human TDG. 10 % SDS-PAGE TDG antibody dilution: 1:10000.Applications for TDG Antibody (622)
Application
Recommended Usage
Western Blot
1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TDG
Long Name
Thymine DNA Glycosylase
Alternate Names
EC 3.2.2.29, thymine-DNA glycosylaseG/T mismatch-specific thymine DNA glycosylase
Gene Symbol
TDG
Additional TDG Products
Product Documents for TDG Antibody (622)
Product Specific Notices for TDG Antibody (622)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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