TDG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20627

Novus Biologicals, part of Bio-Techne
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NBP2-20627
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TDG Antibodies »

Product Summary for TDG Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary.

Reactivity Notes

Rat (84%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TDG Antibody

Knockout Validated: TDG Antibody [NBP2-20627]

Knockout Validated: TDG Antibody [NBP2-20627]

Knockout Validated: TDG Antibody [NBP2-20627] - Wild-type (WT) and TDG knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TDG antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: TDG Antibody [NBP2-20627]

Immunocytochemistry/ Immunofluorescence: TDG Antibody [NBP2-20627]

Immunocytochemistry/Immunofluorescence: TDG Antibody [NBP2-20627] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TDG protein stained by TDG antibody diluted at 1:1000. Blue: Hoechst 33342 staining.
TDG Antibody

Western Blot: TDG Antibody [NBP2-20627] -

Western Blot: TDG Antibody [NBP2-20627] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (NBP2-20627) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Applications for TDG Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol (pH7)

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TDG

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]

Long Name

Thymine DNA Glycosylase

Alternate Names

EC 3.2.2.29, thymine-DNA glycosylaseG/T mismatch-specific thymine DNA glycosylase

Gene Symbol

TDG

UniProt

Q13569 (Human)

Additional TDG Products

Product Documents for TDG Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TDG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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