TDP1 Antibody (PSH0-93)

Western Blot: TDP1 Antibody (PSH0-93) [NBP3-33002]

Western Blot: TDP1 Antibody (PSH0-93) [NBP3-33002] - Western blot analysis of TDP1 on different lysates with Rabbit anti-TDP1 antibody (NBP3-33002) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HeLa cell lysate
Lane 4: A375 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33002) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TDP1 Antibody (PSH0-93) [NBP3-33002]

Immunohistochemistry: TDP1 Antibody (PSH0-93) [NBP3-33002] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TDP1 antibody (NBP3-33002) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33002) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.