TDRKH Antibody (PCRP-TDRKH-1H2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13847
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # PCRP-TDRKH-1H2
Concentration
0.2 mg/ml
Product Summary for TDRKH Antibody (PCRP-TDRKH-1H2)
Immunogen
Recombinant full-length human TDRKH protein (Uniprot: Q9Y2W6 )
Reactivity Notes
Predicted to react with Mouse and Rat.
Localization
Cytoplasm, Localized to cytosol and centrosome
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
62.05 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14276)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for TDRKH Antibody (PCRP-TDRKH-1H2)
Immunocytochemistry/ Immunofluorescence: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847]
Immunocytochemistry/Immunofluorescence: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847] - Immunofluorescence analysis of PFA-fixed HeLa cells. TDRKH Antibody (PCRP-TDRKH-1H2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).Immunohistochemistry-Paraffin: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847]
Immunohistochemistry-Paraffin: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained. TDRKH antibody (PCRP-TDRKH-1H2) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.Flow Cytometry: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847]
Flow Cytometry: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847] - Flow cytometric analysis of PFA-fixed HeLa cells. TDRKH antibody (PCRP-TDRKH-1H2) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).Applications for TDRKH Antibody (PCRP-TDRKH-1H2)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TDRKH
Long Name
Tudor and KH Domain Containing
Alternate Names
TDRD2
Gene Symbol
TDRKH
UniProt
Additional TDRKH Products
Product Documents for TDRKH Antibody (PCRP-TDRKH-1H2)
Product Specific Notices for TDRKH Antibody (PCRP-TDRKH-1H2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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