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TDRKH Antibody (PCRP-TDRKH-1H2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13847

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-13847-100ug
NBP3-13847-20ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # PCRP-TDRKH-1H2

Concentration

0.2 mg/ml

Product Summary for TDRKH Antibody (PCRP-TDRKH-1H2)

Immunogen

Recombinant full-length human TDRKH protein (Uniprot: Q9Y2W6 )

Reactivity Notes

Predicted to react with Mouse and Rat.

Localization

Cytoplasm, Localized to cytosol and centrosome

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

62.05 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14276)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TDRKH Antibody (PCRP-TDRKH-1H2)

Immunocytochemistry/ Immunofluorescence: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847]

Immunocytochemistry/ Immunofluorescence: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847]

Immunocytochemistry/Immunofluorescence: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847] - Immunofluorescence analysis of PFA-fixed HeLa cells. TDRKH Antibody (PCRP-TDRKH-1H2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunohistochemistry-Paraffin: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847]

Immunohistochemistry-Paraffin: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847]

Immunohistochemistry-Paraffin: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained. TDRKH antibody (PCRP-TDRKH-1H2) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Flow Cytometry: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847]

Flow Cytometry: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847]

Flow Cytometry: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847] - Flow cytometric analysis of PFA-fixed HeLa cells. TDRKH antibody (PCRP-TDRKH-1H2) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

Applications for TDRKH Antibody (PCRP-TDRKH-1H2)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TDRKH

Long Name

Tudor and KH Domain Containing

Alternate Names

TDRD2

Gene Symbol

TDRKH

UniProt

Additional TDRKH Products

Product Documents for TDRKH Antibody (PCRP-TDRKH-1H2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TDRKH Antibody (PCRP-TDRKH-1H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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