TdT Antibody (rDNTT/6909) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14198
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rDNTT/6909
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for TdT Antibody (rDNTT/6909) - Azide and BSA Free
Immunogen
Recombinant fragment (around aa1-100) of human TdT protein (exact sequence is proprietary) (Uniprot: P04053)
Localization
Nucleus
Marker
Acute Lymphoblastic Leukemia Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13871).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Applications for TdT Antibody (rDNTT/6909) - Azide and BSA Free
Application
Recommended Usage
ELISA
2-4 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: use this antibody at 2-4ug/ml. For coating, order this antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: TdT
Long Name
Terminal Deoxynucleotidyl Transferase
Alternate Names
DNTT
Gene Symbol
DNTT
Additional TdT Products
Product Documents for TdT Antibody (rDNTT/6909) - Azide and BSA Free
Product Specific Notices for TdT Antibody (rDNTT/6909) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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