TdT Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31368
![Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368]](https://resources.bio-techne.com/images/products/TdT-Antibody-Immunohistochemistry-Paraffin-NBP2-31368-img0002.jpg "Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368]")
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Summary for TdT Antibody
Immunogen
Partial recombinant protein made to an internal sequence of human TdT (between residues 50-300) [UniProt# P04053]
Reactivity Notes
Immunogen's sequence similarity with other species: Rat (77%) Mouse (74%), Bovine (78%)
Localization
Nucleus (high), Cytoplasm (very low)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
58.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TdT Antibody
![Western Blot: TdT Antibody [NBP2-31368]](https://resources.bio-techne.com/images/products/TdT-Antibody-Western-Blot-NBP2-31368-img0001.jpg "Western Blot: TdT Antibody [NBP2-31368]")
Western Blot: TdT Antibody [NBP2-31368]
Western Blot: TdT Antibody [NBP2-31368] - WB detection of TDT protein in (A) lysate of MOLT4 human leukemia cell line and (B) partial recombinant protein by using TDT antibody. Primary antibody concentration used: 1 ug/ml for Molt4 lysate, 0.05 ug/ml for recombinant protein.![Immunocytochemistry/ Immunofluorescence: TdT Antibody [NBP2-31368]](https://resources.bio-techne.com/images/products/TdT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31368-img0006.jpg "Immunocytochemistry/ Immunofluorescence: TdT Antibody [NBP2-31368]")
Immunocytochemistry/ Immunofluorescence: TdT Antibody [NBP2-31368]
Immunocytochemistry/Immunofluorescence: TdT Antibody [NBP2-31368] - TdT antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody concentration of 0.01 ug/ml was used. Image objective 40x.![Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368]](https://resources.bio-techne.com/images/products/TdT-Antibody-Immunohistochemistry-Paraffin-NBP2-31368-img0003.jpg "Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368]")
Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368]
Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368] - IHC-P analysis of TdT protein in a section of human hepatocellular carcinoma (HCC) using TdT antibody at a concentration of 10 ug/ml. The HCC cells depicted strong nuclear / weak cytoplasmic positivity for TdT immunostaining.Applications for TdT Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:100
Immunohistochemistry
10 ug/ml
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
1.0 ug/ml
Application Notes
TdT is a 509 amino acids long protein with predicted molecular weight of 58.5 kDa, and it localizes mainly to the nucleus with low amounts in cytoplasm of the cells. In our Western blot validation with MOLT4 lysate, this TdT antibody detected a single specific band at ~66 kDa position. In our immunohistochemistry validation on formalin fixed paraffin embedded tissue sections, this antibody developed a strong nuclear/ weak cytoplasmic staining in normal lung and hepatocellular carcinoma of human. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TdT
Long Name
Terminal Deoxynucleotidyl Transferase
Alternate Names
DNTT
Entrez Gene IDs
1791 (Human)
Gene Symbol
DNTT
UniProt
Additional TdT Products
Product Documents for TdT Antibody
Product Specific Notices for TdT Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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