Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 200C1339(SB20)

Format

BSA Free

Concentration

1.0 mg/ml

View all TEM8/ANTXR1 Antibodies »

Product Specifications

Immunogen

This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8.

Specificity

Nanda et al (2004) note a passage dependent loss of TEM8 in HUVEC suggesting that that TEM8 may be a specific marker of the endothelial cell (EC) phenotype which is lost as primary EC's age in culture and de-differentiate.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TEM8/ANTXR1 Antibody (200C1339(SB20)) - BSA Free

Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20))BSA Free [NB100-56585]

Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20))BSA Free [NB100-56585]

Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection in TEM8 transfected cell lysate using this antibody.
Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) - BSA Free [NB100-56585]

Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) - BSA Free [NB100-56585]

Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection of TEM8/ANTXR1 in human liver cancer section using 5 ug/ml concentration of TEM8 antibody. Uniform weak staining was observed in the cancerous cells, while the blood vessels depicted the expected intense positivity for TEM8.
Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20))BSA Free [NB100-56585]

Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20))BSA Free [NB100-56585]

Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Analysis of human heart lysates (35ug per lane, RIPA buffer) using TEM8 antibody (NB100-56585) at 0.3ug/ml. Band observed at 65kDa. (Expected MW of 62.8kDa according to NP_115584.1).
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Applications for TEM8/ANTXR1 Antibody (200C1339(SB20)) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (Matsuda (2010))

Immunohistochemistry

2-5 ug/ml

Immunohistochemistry-Paraffin

2-5 ug/ml. Use reported in scientific literature (Hotchkiss et al (2005))

Western Blot

1-3 ug/ml
Application Notes
TEM8 is observed at ~80 kDa in western blots of HUVEC cells (Hotchkiss et al, 2005) and as an 80 kDa and 85 kDa double in human colon cell lysates (Nanada et al, 2004). The size difference is thought to be due to glycosylation as a 70 kDa band was seen when cell extracts in Nanda et al (2004) were treated with a glycosidase cocktail.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TEM8/ANTXR1

Recently, using SAGE (Serial Analysis of Gene Expression) technology, St. Croix et al, have identified 46 genes, whose expression is specifically elevated in tumor-associated endothelium. Nine of these genes were prominently expressed only in tumor endothelial cells (EC), but were absent or barely detectable in normal ECs, and named as Tumor Endothelial Markers (TEMs, TEM 1-9). Based on TEM8s interaction with the C5 domain of collagen a3(VI), a protein also preferentially expressed in tumor endothelium, it is thought that TEM8 plays an important role in tumor angiogenesis.

Long Name

Tumor Endothelial Marker 8/Anthrax Toxin Receptor 1

Alternate Names

ANTXR1

Entrez Gene IDs

84168 (Human); 69538 (Mouse); 362393 (Rat)

Gene Symbol

ANTXR1

UniProt

Q9H6X2

Additional TEM8/ANTXR1 Products

Product Documents for TEM8/ANTXR1 Antibody (200C1339(SB20)) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TEM8/ANTXR1 Antibody (200C1339(SB20)) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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