Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # T2H5

Format

BSA Free

Concentration

1.0 mg/ml

View all Tenascin C Antibodies »

Product Specifications

Immunogen

Protein preparation from a homogenate of a human mammary tumour specimen.

Specificity

The antibody T2H5 recognizes tenascin, a large hexameric extracellular matrix glycoprotein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Tenascin C Antibody (T2H5) - BSA Free

Immunohistochemistry-Paraffin: Tenascin C Antibody (T2H5) - BSA Free [NBP3-18002]

Immunohistochemistry-Paraffin: Tenascin C Antibody (T2H5) - BSA Free [NBP3-18002]

Immunohistochemistry-Paraffin: Tenascin C Antibody (T2H5) - BSA Free [NBP3-18002] - Staining of tonsil

Applications for Tenascin C Antibody (T2H5) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:50

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemical detection of tenascin is valuable for studies of tissue differentiation and tumour growth. The antibody T2H5 is excellent for staining of paraffin-embedded tissue sections.Heat-mediated antigen retrieval in citrate buffer

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH 8.0)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Tenascin C

Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle. Tenascin (TN)(Erickson and Bourdon 1989, Erickson 1993) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1-200/220, myotendinous antigen (MI), neuronectin (NEC1) and glioma mesenchymal extracellular matrix (GMEM). The TN molecule is a disulfide-linked hexamer. Human TN has 3 subunits of 190, 200 and 220 kDa. It is primarily made up of 14.5 epidermal growth-factor-like repeats, 15 units similar to the fibronectin type-III-homology repeat and, at the C-terminus, has a sequence with homology to the globular domain of the beta and gamma chain of fibrinogen. Monoclonal antibody reacting specifically with tenascin is an essential tool for the localization, identification and studies on the role of the molecule in epithelial-mesenchymal and neuronal-glial interactions (Castellucci et al. 1991, Balza et al. 1993).

Alternate Names

Cytotactin, HXB, Tenascin J1, TNC

Gene Symbol

TNC

Additional Tenascin C Products

Product Documents for Tenascin C Antibody (T2H5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Tenascin C Antibody (T2H5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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