Tenascin X Antibody (2D3-1B10)

Novus Biologicals, part of Bio-Techne | Catalog # H00007148-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2D3-1B10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Tenascin X Antibodies »

Product Specifications

Immunogen

TNXB (AAH33740, 1 a.a. ~ 673 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRLSWSVAQGPFDSFVVQYEDTNGQPQALLVDGDQSKILISGLEPSTPYRFLLYGLHEGKRLGPLSAEGTTGLAPAGQTSEESRPRLSQLSVTDVTTSSLRLNWEAPPGAFDSFLLRFGVPSPSTLEPHPRPLLQRELMVPGTRHSAVLRDLRSGTLYSLTLYGLRGPHKADSIQGTARTLSPVLESPRDLQFSEIRETSAKVNWMPPPSRADSFKVSYLLADGGEPQSVQVDGQARTQKLQGLIPGARYEVTVVSVRGFEESEPLTGFLTTVPDGPTQLRALNLTEGFAVLHWKPPQNPVDTYDVQVTAPGAPPLQAETPGSAVDYPLHDLVLHTNYTATVRGLRGPNLTSPASITFTTGLEAPRDLEAKEVTPRTALLTWTEPPVRPAGYLLSFHTPGGQNQEILLPGGITSHQLLGLFPSTSYNARLQAMWGQSLLPPVSTSFTTGGLRIPFPRDCGEEMQNGAGASRTSTIFLNGNRERPLNVFCDMETDGGGWLVFQRRMDGQTDFWRDWEDYAHGFGNISGEFWLGNEALHSLTQAGDYSMRVDLRAGDEAVFAQYDSFHVDSAAEYYRLHLEGYHGTAGDSMSYHSGSVFSARDRDPNSLLISCAVSYRGAWWYRNCHYANLNGLYGSTVDHQGVSWYHWKGFEFSVPFTEMKLRPRNFRSPAGGG

Specificity

TNXB - tenascin XB

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Tenascin X Antibody (2D3-1B10)

Western Blot: Tenascin X Antibody (2D3-1B10) [H00007148-M01]

Western Blot: Tenascin X Antibody (2D3-1B10) [H00007148-M01]

Western Blot: Tenascin X Antibody (2D3-1B10) [H00007148-M01] - Analysis of TNXB expression in transfected 293T cell line by TNXB monoclonal antibody (M01), clone 2D3-1B10.Lane 1: TNXB transfected lysate (Predicted MW: 74 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: Tenascin X Antibody (2D3-1B10) [H00007148-M01]

Immunoprecipitation: Tenascin X Antibody (2D3-1B10) [H00007148-M01]

Immunoprecipitation: Tenascin X Antibody (2D3-1B10) [H00007148-M01] - Analysis of TNXB transfected lysate using anti-TNXB monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TNXB MaxPab rabbit polyclonal antibody.
Sandwich ELISA: Tenascin X Antibody (2D3-1B10) [H00007148-M01] - Detection limit for recombinant GST tagged TNXB is approximately 0.03ng/ml as a capture antibody.

Applications for Tenascin X Antibody (2D3-1B10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Tenascin X

This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The tenascins have anti-adhesive effects, as opposed to fibronectin which is adhesive. This protein is thought to function in matrix maturation during wound healing, and its deficiency has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. It is one of four genes in this cluster which have been duplicated. The duplicated copy of this gene is incomplete and is a pseudogene which is transcribed but does not encode a protein. The structure of this gene is unusual in that it overlaps the CREBL1 and CYP21A2 genes at its 5' and 3' ends, respectively. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternate Names

HXBL, TNX, TNXB, TNXBS, XB, XB-S

Entrez Gene IDs

7148 (Human)

Gene Symbol

TNXB

OMIM

130020 (Human)

UniProt

AAH33740

Additional Tenascin X Products

Product Documents for Tenascin X Antibody (2D3-1B10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Tenascin X Antibody (2D3-1B10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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