Tensin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84129

Novus Biologicals, part of Bio-Techne
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NBP1-84129
NBP1-84129-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Tensin 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYK

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33534863). Rat reactivity reported from a verified customer review (staining of rat kidneys shows positivity in mesangial cells).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Tensin 1 Antibody

Immunocytochemistry/ Immunofluorescence: Tensin 1 Antibody [NBP1-84129]

Immunocytochemistry/ Immunofluorescence: Tensin 1 Antibody [NBP1-84129]

Immunocytochemistry/Immunofluorescence: Tensin 1 Antibody [NBP1-84129] - Adhesions are more mature and stable on PT compared to SLA. Co-localizations of tensin-1 and p-cortactin with vinculin were shown by immunocytochemical staining of HGFs cultured on PT and SLA surfaces at 24 hrs. Cells were labelled for integrin tensin-1 (red), vinculin (green), and nuclei (blue). Representative images of HGFs on PT and SLA observed under fluorescence microscopy. Grey scale images are shown for separate channels for tensin-1, and vinculin. Inserts show higher magnifications of focal contacts (white boxes). Image collected and cropped by CiteAb from the following publication (https://doi.wiley.com/10.1111/jcmm.12451), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84129]

Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84129]

Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84129] - Staining of rat kidneys shows positivity in mesangial cells. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84129]

Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84129]

Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84129] - Staining of human liver shows moderate membranous positivity in hepatocytes.
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Applications for Tensin 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reactivity reported in (PMID: 25766369).

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

Reported in scientific literature (PMID: 30813060).
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP1-84129 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tensin 1

Tensin 1 (TNS1, human Tensin 1 theoretical molecular weight 186kDa) belongs to a family of focal adhesion proteins which in mammals includes three other members: Tensin 2, Tensin 3, and C-terminal tensin-like (Cten or Tensin 4) (1, 2). Tensins localize to focal adhesion sites, which are functional domains of the cellular membrane that mediate interactions between the actin cytoskeleton and the extracellular matrix (1). Transmembrane integrin receptors (alpha-beta heterodimer) predominate within focal adhesions and serve to bridge the interactions between extracellular components and the cytoskeleton (3). Tensin 1 interacts with both actin filaments and beta-integrin receptors within focal adhesion sites to mediate cellular responses to extracellular signals (1, 4). Several common functional domains are shared between some tensin family members including: 1) amino terminal PTEN-related tyrosine phosphatase (PTP) domain, 2) amino terminal actin-binding (ABD-1) domain, 3) amino terminal focal adhesion binding (FAB-N) domain, 4) carboxy terminal Src homology 2 (SH2) domain, 5) carboxy terminal phosphotyrosine binding (PTB) domain, and 6) carboxy terminal focal adhesion binding (FAB-C) domain (4). The PTB domain allows tensins to interact with beta-integrin cytoplasmic tails, while their SH2 domain supports interaction with tyrosine-phosphorylated signaling proteins (1). The interactions of tensins with focal adhesion kinases and phosphatases support diverse cellular processes such as attachment, migration, and polarization. Loss of function studies have revealed that tensin 1 plays a critical role for normal kidney function, skeletal muscle regeneration and angiogenesis (1).

References

1. Lo, S. H. (2017). Tensins. Current Biology. https://doi.org/10.1016/j.cub.2017.02.041

2. Lo, S. H. (2014). C-terminal tensin-like (CTEN): A promising biomarker and target for cancer. International Journal of Biochemistry and Cell Biology. https://doi.org/10.1016/j.biocel.2014.04.003

3. Armitage, S. K., & Plotnikov, S. V. (2016). Bridging the gap: A new study reveals that a protein called talin forms a vital link between microtubules and focal adhesions at the surface of cells. ELife. https://doi.org/10.7554/eLife.19733

4. Lo, S. H. (2006). Focal adhesions: What's new inside. Developmental Biology. https://doi.org/10.1016/j.ydbio.2006.03.029

Alternate Names

DKFZp586K0617, matrix-remodelling associated 6, Matrix-remodelling-associated protein 6, MGC88584, MST091, MST122, MST127, MSTP127, MXRA6, tensin, tensin 1, tensin-1, TNSMSTP122

Gene Symbol

TNS1

Additional Tensin 1 Products

Product Documents for Tensin 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tensin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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