Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

FISH, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TERF2IP Antibodies »

Product Specifications

Immunogen

Antibody was generated using recombinant full length human RAP1 (Telomeric repeat binding factor 2 interacting protein 1) according to entry NP_061848.2

Localization

Telomeres

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TERF2IP Antibody

Western Blot: TERF2IP Antibody [NB100-292]

Western Blot: TERF2IP Antibody [NB100-292]

Western Blot: TERF2IP Antibody [NB100-292] - Detection of Human RAP1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP1 antibody NB100-292 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: TERF2IP Antibody [NB100-292]

Immunohistochemistry-Paraffin: TERF2IP Antibody [NB100-292]

Immunohistochemistry-Paraffin: TERF2IP Antibody [NB100-292] - Sections of human lung carcinoma.~~ Antibody: Affinity purified rabbit anti-RAP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: TERF2IP Antibody [NB100-292]

Immunoprecipitation: TERF2IP Antibody [NB100-292]

Immunoprecipitation: TERF2IP Antibody [NB100-292] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-RAP1 antibody used for IP at 6 ug per reaction. RAP1 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-RAP1 antibody. For blotting immunoprecipitated RAP1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for TERF2IP Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2:000

Immunohistochemistry-Paraffin

1:500 - 1:2:000

Immunoprecipitation

1:250 - 1:1000

Western Blot

1:500-1:5000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. ICC/IF reactivity reported in scientific literature (PMID: 25271372).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TERF2IP

Mammalian telomeric proteins, including TRF1, TRF2, tankyrase, and TERF21P have no recognized orthologs in budding yeast. However, human Rap1 (hRap1) is an ortholog of the yeast telomeric protein, scRap1p. hRap1 is a homodimer, has three conserved sequence motifs in common with scRap1, is located at telomeres, and plays a role in telomere length regulation. Although scRap1 binds telomeric DNA directly, TERF21P is recruited to telomeres by TERF21P and binds it with its C-terminus, rather than directly binding DNA.

Alternate Names

dopamine receptor interacting protein 5, Dopamine receptor-interacting protein 5, DRIP5, hRap1, RAP1 homolog, RAP1TERF2-interacting telomeric protein 1, Repressor/activator protein 1 homolog, telomeric repeat binding factor 2, interacting protein, telomeric repeat-binding factor 2-interacting protein 1, TRF2-interacting telomeric protein 1, TRF2-interacting telomeric RAP1 protein

Entrez Gene IDs

54386 (Human)

Gene Symbol

TERF2IP

UniProt

Q9NYB0 (Human)

Additional TERF2IP Products

Product Documents for TERF2IP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TERF2IP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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