TERF2IP Antibody

Western Blot: TERF2IP Antibody [NB100-56321]

Western Blot: TERF2IP Antibody [NB100-56321] - Total protein from HeLa, SHSH-5Y, IMR-90 and MCF7 cells were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TERF2IP in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/ Immunofluorescence: TERF2IP Antibody [NB100-56321]

Immunocytochemistry/Immunofluorescence: TERF2IP Antibody [NB100-56321] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-TERF2IP [NB100-56321] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A; Cat# NB100-690) was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: TERF2IP Antibody [NB100-56321]

Western Blot: TERF2IP Antibody [NB100-56321] - TERF21P Antibody [NB100-56321] - 2 ug of this antibody was used to detect hRap1 in 293 cells. A protein band with an approximate molecular weight of 50 kDa was detected.