TERT Antibody (2D8)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-297

Novus Biologicals, part of Bio-Techne
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NB100-297
NB100-297SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 2D8

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all TERT Antibodies »

Product Specifications

Immunogen

Full-length recombinant human Telomerase reverse transcriptase from insect cells. [UniProt# O14746]

Localization

Nuclear

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Theoretical MW

127 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TERT Antibody (2D8)

Western Blot: TERT Antibody (2D8) [NB100-297]

Western Blot: TERT Antibody (2D8) [NB100-297]

Western Blot: TERT Antibody (2D8) [NB100-297] - Analysis of human TERT, using NB100-297. Sample: MJ90 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: TERT Antibody (2D8) [NB100-297]

Immunocytochemistry/ Immunofluorescence: TERT Antibody (2D8) [NB100-297]

Immunocytochemistry/Immunofluorescence: TERT Antibody (2D8) [NB100-297] - Detection of Tert (green) in HepG2 cells using NB100-297. Nuclei (Blue) are counterstained with Hoechst 33258.
Immunohistochemistry-Paraffin: TERT Antibody (2D8) [NB100-297]

Immunohistochemistry-Paraffin: TERT Antibody (2D8) [NB100-297]

Immunohistochemistry-Paraffin: TERT Antibody (2D8) [NB100-297] - TERT was detected in immersion fixed paraffin-embedded sections of human liver cancer using anti-human mouse monoclonal antibody (Catalog # NB100-297) at 1:600 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).
Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

Applications for TERT Antibody (2D8)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Frozen

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000
Application Notes
In Western blot, this antibody recognizes a band at ~127 kDa and additional non-specific background band ~110 kDa may also be seen. Note that the isotype is IgM and the appropriate secondary should be used. For immunopurification of telomerase, please see the procedure guide for a detailed protocol.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TERT

Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication that is active in progenitor as well as cancer cells, and stays inactive or show very low activity in normal somatic cells. TERT is the catalytic component of the holoenzyme complex (TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) that implicates in telomeres elongation by acting as a reverse transcriptase adding simple sequence repeats to chromosome ends by copying a template sequence within RNA component of the enzyme. For executing its versatile functions, TERT can interact with HSP90A , PTGES3, HSPA1A, RAN , XPO1, PTPN11, NCL, SMARCA4, MCRS1, PIF1, PML and GNL3L. TERT modulates Wnt signaling and plays important roles in aging and anti-apoptosis. Telomerase activation has been implicated in cell immortalization/ carcinogenesis and defects in TERT are associated with susceptibilty to aplastic anemia, coronary artery disease (CAD), dyskeratosis congenita autosomal dominant type 2 (DKCA2), pulmonary fibrosis, and/or bone marrow failure, telomere-related, type 1 (PFBMFT1), dyskeratosis congenita autosomal recessive type 4 (DKCB4), susceptibility to pulmonary fibrosis idiopathic (IPF).

Long Name

Telomerase Reverse Transcriptase

Alternate Names

EST2Telomerase-associated protein 2, hEST2, hTERT, TCS1EC 2.7.7.49, Telomerase catalytic subunit, telomerase reverse transcriptase, TP2HEST2, TRTEC 2.7.7

Entrez Gene IDs

7015 (Human)

Gene Symbol

TERT

UniProt

O14746

Additional TERT Products

Product Documents for TERT Antibody (2D8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TERT Antibody (2D8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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