Testin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87987

Novus Biologicals, part of Bio-Techne
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NBP1-87987
NBP1-87987-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human, Canine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Testin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QLPAHDQDPSKCHELSPREVKEMEQFVKKYKSEALGVGDVKLPCEMDAQGPKQMNIPGGDRSTPAAVGAMEDKSAEHKRTQYSCYCCKLSMKEGDPAIYAERAGYDKLWHPACFVCSTCHELLVD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (85%). Reactivity reported in scientific literature (PMID: 23227862)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Testin Antibody

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human endometrium, liver, testis and urinary bladder using Anti-TES antibody NBP1-87987 (A) shows similar protein distribution across tissues to independent antibody NBP1-85072 (B).
Western Blot: Testin Antibody [NBP1-87987]

Western Blot: Testin Antibody [NBP1-87987]

Western Blot: Testin Antibody [NBP1-87987] - Analysis in human cell lines A-431 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Western Blot: Testin Antibody [NBP1-87987]

Western Blot: Testin Antibody [NBP1-87987]

Western Blot: Testin Antibody [NBP1-87987] - Analysis using Anti-TES antibody NBP1-87987 (A) shows similar pattern to independent antibody NBP1-85072 (B).
View 9 more images

Applications for Testin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

reported by internal validation

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in IHC-P reported in scientific literature (PMID:34440214).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Testin

Cancer-associated chromosomal changes often involve regions containing fragile sites. This gene maps to a commom fragile site on chromosome 7q31.2 designated FRA7G. This gene is similar to mouse Testin, a testosterone-responsive gene encoding a Sertoli cell secretory protein containing three LIM domains. LIM domains are double zinc-finger motifs that mediate protein-protein interactions between transcription factors, cytoskeletal proteins and signaling proteins. Multiple protein isoforms are encoded by transcript variants of this gene.

Alternate Names

DKFZP586B2022, MGC1146, TESS, TESS-2, testin, testis derived transcript (3 LIM domains)

Gene Symbol

TES

Additional Testin Products

Product Documents for Testin Antibody

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Testin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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