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TET2 Antibody (CL6873) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44224

Novus Biologicals, part of Bio-Techne
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NBP3-44224

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL6873

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q6N021, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for TET2 Antibody (CL6873) - Azide and BSA Free

TET2 Antibody (CL6873) - Azide and BSA Free Immunohistochemistry-Paraffin: TET2 Antibody [NBP3-44224]

Immunohistochemistry-Paraffin: TET2 Antibody [NBP3-44224]

Analysis in human placenta and pancreas tissues using NBP3-44224 antibody. Corresponding TET2 RNA-seq data are presented for the same tissues.
TET2 Antibody (CL6873) - Azide and BSA Free Immunohistochemistry-Paraffin: TET2 Antibody [NBP3-44224]

Immunohistochemistry-Paraffin: TET2 Antibody [NBP3-44224]

Staining of human pancreas shows no nuclear positivity in exocrine glandular cells as expected.
TET2 Antibody (CL6873) - Azide and BSA Free Immunohistochemistry-Paraffin: TET2 Antibody [NBP3-44224]

Immunohistochemistry-Paraffin: TET2 Antibody [NBP3-44224]

Staining of human placenta shows moderate nuclear positivity in a subset of trophoblastic cells.

Applications for TET2 Antibody (CL6873) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TET2

Long Name

Methylcytosine dioxygenase TET2

Alternate Names

KIAA1546, Nbla00191

Gene Symbol

TET2

Additional TET2 Products

Product Documents for TET2 Antibody (CL6873) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TET2 Antibody (CL6873) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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