TEV Protease Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97669

Novus Biologicals, part of Bio-Techne
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NBP1-97669
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Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Virus - HPV

Applications

Validated:

ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TEV Protease Antibodies »

Product Specifications

Immunogen

This protein-A purified TEV Protease Antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant MBP-and-poly-His-tagged auto inactivation-resistant mutant TEV Protease. (Uniprot: P04517)

Reactivity Notes

Reactive with Tobacco Etch Virus

Specificity

Assay by western blot showed no reactivity to MBP.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for TEV Protease Antibody

Western Blot: TEV Protease Antibody [NBP1-97669]

Western Blot: TEV Protease Antibody [NBP1-97669]

Western Blot: TEV Protease Antibody [NBP1-97669] - No translocation was observed for TEV protease cargo alone. Western blots show the presence of the constructs at lower retention volumes during size exclusion chromatography both at pH 8 (Left) and pH 11 (Right), meaning they co-localize with the rest of the holotoxin even after prepore-to-pore transition and are not translocated. Note: Lower retention volume towards left of blot and higher retention volume toward right. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31748551/) licensed under a CC-BY license.
Western Blot: TEV Protease Antibody [NBP1-97669]

Western Blot: TEV Protease Antibody [NBP1-97669]

Western Blot: TEV Protease Antibody [NBP1-97669] - E.coli lysate containing recombinant TEV protease, NBP1-97669 used at 1:1000 dilution.

Applications for TEV Protease Antibody

Application
Recommended Usage

ELISA

1:1500-1:27000

Western Blot

1:500
Application Notes
This protein A purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kDa in size corresponding to TEV by western blotting in the appropriate cell lysate or extract. Anti-TEV protease may be useful to detect residual TEV protease in preparations of recombinant proteins in which that protease may interfere with downstream manipulations.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: TEV Protease

TEV protease, encoded by the Tobacco Etch Virus (TEV), is a 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the virus (TEV). It is widely used for cleaving fusion proteins because of its sequence specificity. It recognizes a linear epitope of the general form E-Xaa-Xaa-Y-Xaa-Q-(G/S). Cleavage occurs between Q and G or Q and S. The structure of TEV protease is similar to that of of the serine protease family. Like serine proteases, TEV protease utilizes a catalytic triad of residues to hydrolyze peptide bonds. The distinguishing feature of TEV protease, however, is that instead of the serine nucleophile in the triad Ser-Asp-His, there is a cysteine, which may explain the resistance of TEV protease to protease inhibitors which are commonly used. The strain used is the autoinactivation-resistant mutant S219V. The catalytic activity of the S219V mutant is approximately 2 fold greater than that of the wild-type protease.

Long Name

Tobacco Etch Virus Protease

Alternate Names

NIa

Gene Symbol

TEVgp1

UniProt

P04517 (Virus)

Additional TEV Protease Products

Product Documents for TEV Protease Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TEV Protease Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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