TFAP4 Antibody (6B1)

Novus Biologicals, part of Bio-Techne | Catalog # H00007023-M01

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6B1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

TFAP4 (NP_003214, 93 a.a. ~ 192 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSPDIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIA

Specificity

TFAP4 (6B1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TFAP4 Antibody (6B1)

Western Blot: TFAP4 Antibody (6B1) [H00007023-M01]

Western Blot: TFAP4 Antibody (6B1) [H00007023-M01] - TFAP4 monoclonal antibody (M01), clone 6B1 Analysis of TFAP4 expression in Hela S3 NE.

Immunocytochemistry/ Immunofluorescence: TFAP4 Antibody (6B1) [H00007023-M01]

Immunocytochemistry/Immunofluorescence: TFAP4 Antibody (6B1) [H00007023-M01] - Analysis of monoclonal antibody to TFAP4 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: TFAP4 Antibody (6B1) [H00007023-M01]

Immunohistochemistry-Paraffin: TFAP4 Antibody (6B1) [H00007023-M01] - Analysis of monoclonal antibody to TFAP4 on formalin-fixed paraffin-embedded human uterine cervix. Antibody concentration 1 ug/ml.

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Applications for TFAP4 Antibody (6B1)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TFAP4

Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 (PubMed 2833704); Hu et al., 1990 (PubMed 2123466)).(supplied by OMIM)

Alternate Names

Activating enhancer-binding protein 4, AP-4, BHLHC41, bHLHc41Class C basic helix-loop-helix protein 41, transcription factor AP-4, transcription factor AP-4 (activating enhancer binding protein 4), transcription factor AP-4 (activating enhancer-binding protein 4)

Entrez Gene IDs

7023 (Human)

Gene Symbol

TFAP4

OMIM

600743 (Human)

UniProt

NP_003214 (Human)

Additional TFAP4 Products

Product Documents for TFAP4 Antibody (6B1)

Product Datasheets

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COA

SDS

Product Specific Notices for TFAP4 Antibody (6B1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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