TFAP4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35696
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-338 of human TFAP4 (NP_003214.1).
Sequence:
QQEQVRLLHQEKLEREQQQLRTQLLPPPAPTHHPTVIVPAPPPPPSHHINVVTMGPSSVINSVSTSRQNLDTIVQAIQHIEGTQEKQELEEEQRRAVIVKPVRSCPEAPTSDTASDSEASDSDAMDQSREEPSGDGELP
Sequence:
QQEQVRLLHQEKLEREQQQLRTQLLPPPAPTHHPTVIVPAPPPPPSHHINVVTMGPSSVINSVSTSRQNLDTIVQAIQHIEGTQEKQELEEEQRRAVIVKPVRSCPEAPTSDTASDSEASDSDAMDQSREEPSGDGELP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TFAP4 Antibody
Immunocytochemistry/ Immunofluorescence: TFAP4 Antibody [NBP3-35696] -
Immunocytochemistry/ Immunofluorescence: TFAP4 Antibody [NBP3-35696] - Immunofluorescence analysis of C6 cells using [KO Validated] TFAP4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western Blot: TFAP4 Antibody [NBP3-35696] -
Western Blot: TFAP4 Antibody [NBP3-35696] - Western blot analysis of various lysates using [KO Validated] TFAP4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: TFAP4 Antibody [NBP3-35696] -
Immunocytochemistry/ Immunofluorescence: TFAP4 Antibody [NBP3-35696] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] TFAP4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for TFAP4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:200 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TFAP4
Alternate Names
Activating enhancer-binding protein 4, AP-4, BHLHC41, bHLHc41Class C basic helix-loop-helix protein 41, transcription factor AP-4, transcription factor AP-4 (activating enhancer binding protein 4), transcription factor AP-4 (activating enhancer-binding protein 4)
Gene Symbol
TFAP4
Additional TFAP4 Products
Product Documents for TFAP4 Antibody
Product Specific Notices for TFAP4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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