TFEB Antibody (BLR070G)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77360
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # BLR070G
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Immunogen was a peptide representing a region between residue 426 and the C-terminus (residue 476) of human Transcription factor EB using the numbering given in entry NP_009093.1 (Gene ID 7942).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TFEB Antibody (BLR070G)
Western Blot: TFEB Antibody (BLR070G) [NBP2-77360]
Western Blot: TFEB Antibody (BLR070G) [NBP2-77360] - Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1,and BW5147.3 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-TFEB recombinant monoclonalantibody [BLR070G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].Western Blot: TFEB Antibody (BLR070G) [NBP2-77360]
Western Blot: TFEB Antibody (BLR070G) [NBP2-77360] - Whole cell lysate (50 ug) from HeLa, Hep-G2, MCF-7, Jurkat, andK-562 cells prepared using NETN lysis buffer. Antibody:Rabbit anti-TFEB recombinant monoclonal antibody[BLR070G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG.Chemiluminescence with an exposure time of 3 minutes.Lower Panel: Rabbit anti-Actin recombinant monoclonalantibody [BLR057F].Applications for TFEB Antibody (BLR070G)
Application
Recommended Usage
Immunoprecipitation
20 ul/mg lysate
Western Blot
1:1000
Application Notes
Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: TFEB
Long Name
Transcription Factor EB
Alternate Names
ALPHATFEB, BHLHE35, TCFEB
Gene Symbol
TFEB
Additional TFEB Products
Product Documents for TFEB Antibody (BLR070G)
Product Specific Notices for TFEB Antibody (BLR070G)
This Product sold under License from Bethyl Laboratories, Inc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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