TFEB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93447

Novus Biologicals, part of Bio-Techne
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NB100-93447
NB100-93447-20ug
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all TFEB Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QKDLQKSRELENH corresponding to internal region according to NP_009093.1.

Epitope

C-QKDLQKSRELENH

Specificity

This antibody is expected not to cross-react to TFE3 and MITF.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TFEB Antibody

Western Blot: TFEB Antibody [NB100-93447]

Western Blot: TFEB Antibody [NB100-93447]

Western Blot: TFEB Antibody [NB100-93447] - Representative western blots of Atg7, TFEB and p62 at different times after LPS injection. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-08722-3), licensed under a CC-BY license.
Western Blot: TFEB Antibody [NB100-93447]

Western Blot: TFEB Antibody [NB100-93447]

Western Blot: TFEB Antibody [NB100-93447] - (1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for TFEB Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:1000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in human lung lysates (calculated MW of 52.9 kDa band according to NP_009093.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TFEB

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta

Long Name

Transcription Factor EB

Alternate Names

ALPHATFEB, BHLHE35, TCFEB

Entrez Gene IDs

7942 (Human); 21425 (Mouse); 316214 (Rat)

Gene Symbol

TFEB

UniProt

P19484

Additional TFEB Products

Product Documents for TFEB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TFEB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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