Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TFEB Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 325 and 375 of human Transcription Factor EB using the numbering given in entry NP_009093.1 (GeneID 7942).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFEB Antibody

Western Blot: TFEB Antibody [NBP2-12758]

Western Blot: TFEB Antibody [NBP2-12758]

Western Blot: TFEB Antibody [NBP2-12758] - Detection of Human TFEB by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody NBP2-12758 used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 10 second.
Immunoprecipitation: TFEB Antibody [NBP2-12758]

Immunoprecipitation: TFEB Antibody [NBP2-12758]

Immunoprecipitation: TFEB Antibody [NBP2-12758] - Detection of human TFEB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TFEB antibody NBP2-12758 used for IP at 6 ug per reaction. TFEB was also immunoprecipitated by a different rabbit anti-TFEB antibody and rabbit anti-TFEB recombinant monoclonal antibody (NBP2-77360). For blotting immunoprecipitated TFEB, NBP2-77360 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for TFEB Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TFEB

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta

Long Name

Transcription Factor EB

Alternate Names

ALPHATFEB, BHLHE35, TCFEB

Gene Symbol

TFEB

UniProt

NP_009093.1 (Human)

Additional TFEB Products

Product Documents for TFEB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TFEB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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