TFEC Antibody (4F11)

Novus Biologicals, part of Bio-Techne | Catalog # H00022797-M08

Novus Biologicals, part of Bio-Techne
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H00022797-M08
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4F11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TFEC Antibodies »

Product Specifications

Immunogen

TFEC (NP_001018068.1, 1 a.a. ~ 89 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTLDHQIINPTLKWSQPAVPSGGPLVQHAHTTLDSDAGLTENPLTKLLAIGKEDDNAQWHLSGSILDVYSGEQGISPINMGLTSASCPS

Specificity

TFEC - transcription factor EC (4F11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for TFEC Antibody (4F11)

Western Blot: TFEC Antibody (4F11) [H00022797-M08]

Western Blot: TFEC Antibody (4F11) [H00022797-M08]

Western Blot: TFEC Antibody (4F11) [H00022797-M08] - Analysis of TFEC expression in transfected 293T cell line by TFEC monoclonal antibody (M08), clone 4F11. Lane 1: TFEC transfected lysatE (22.7 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: TFEC Antibody (4F11) [H00022797-M08] - Detection limit for recombinant GST tagged TFEC is 0.1 ng/ml as a capture antibody.

Applications for TFEC Antibody (4F11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TFEC

Transcriptional regulator that acts as a repressor or an activator. Acts as a transcriptional repressor on minimal promoter containing element F (that includes an E-box sequence). Binds to element F in an E-box sequence-specific manner. Acts as a transcriptional transactivator on the proximal promoter region of the tartrate-resistant acid phosphatase (TRAP) E-box containing promoter . Collaborates with MITF in target gene activation . Acts as a transcriptional repressor on minimal promoter containing mu E3 enhancer sequence . Binds to mu E3 DNA sequence of the immunoglobulin heavy-chain gene enhancer . Binds DNA in a homo- or heterodimeric form

Alternate Names

Class E basic helix-loop-helix protein 34, hTFEC-L, TCFECTFECLbHLHe34BHLHE34, TFE-C, transcription factor EC, Transcription factor EC-like

Entrez Gene IDs

22797 (Human)

Gene Symbol

TFEC

UniProt

NP_001018068 (Human)

Additional TFEC Products

Product Documents for TFEC Antibody (4F11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TFEC Antibody (4F11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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