TFF1/pS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16376

Novus Biologicals, part of Bio-Techne
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NBP2-16376
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TFF1 Antibodies »

Product Summary for TFF1/pS2 Antibody

Immunogen

Full length human TFF1/pS2 Recombinant protein.

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TFF1/pS2 Antibody

Western Blot: TFF1/pS2 Antibody [NBP2-16376]

Western Blot: TFF1/pS2 Antibody [NBP2-16376]

Western Blot: TFF1/pS2 Antibody [NBP2-16376] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with TFF1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: TFF1/pS2 Antibody [NBP2-16376]

Immunocytochemistry/ Immunofluorescence: TFF1/pS2 Antibody [NBP2-16376]

Immunocytochemistry/Immunofluorescence: TFF1/pS2 Antibody [NBP2-16376] - Analysis of methanol-fixed MCF-7, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: TFF1/pS2 Antibody [NBP2-16376]

Immunohistochemistry-Paraffin: TFF1/pS2 Antibody [NBP2-16376]

Immunohistochemistry-Paraffin: TFF1/pS2 Antibody [NBP2-16376] - Human gastric cancer, using Trefoil factor 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Applications for TFF1/pS2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TFF1

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq]

Long Name

Trefoil Factor 1

Alternate Names

BCEI, D21S21, HPS2, PNR-2, PS2

Gene Symbol

TFF1

UniProt

P04155

Additional TFF1 Products

Product Documents for TFF1/pS2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TFF1/pS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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