TFF3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34007

Novus Biologicals, part of Bio-Techne
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NBP2-34007
NBP2-34007-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TFF3 Antibodies »

Product Summary for TFF3 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SSAEEYVGLSANQCAVPAKDRVDCGYPHVTPKECNNRGCCFDSRIPGVPWCFKPLQEAECT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFF3 Antibody

Immunocytochemistry/ Immunofluorescence: TFF3 Antibody [NBP2-34007]

Immunocytochemistry/ Immunofluorescence: TFF3 Antibody [NBP2-34007]

Immunocytochemistry/Immunofluorescence: TFF3 Antibody [NBP2-34007] - Staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: TFF3 Antibody [NBP2-34007]

Immunohistochemistry-Paraffin: TFF3 Antibody [NBP2-34007]

Immunohistochemistry-Paraffin: TFF3 Antibody [NBP2-34007] - Staining of human colon shows moderate to strong positivity in mucus in glandular cells.
Immunohistochemistry-Paraffin: TFF3 Antibody [NBP2-34007]

Immunohistochemistry-Paraffin: TFF3 Antibody [NBP2-34007]

Immunohistochemistry-Paraffin: TFF3 Antibody [NBP2-34007] - Staining of human small intestine shows distinct positivity in goblet cells.
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Applications for TFF3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFF3

Trefoil Factor 3 is a member of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. Trefoil Factor 3 and two other related trefoil family member genes are found in a cluster on chromosome 21.

Long Name

Trefoil Factor 3

Alternate Names

ITF, TFI

Gene Symbol

TFF3

UniProt

Q07654

Additional TFF3 Products

Product Documents for TFF3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TFF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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