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TFF3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13358

Novus Biologicals, part of Bio-Techne
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NBP3-13358

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TFF3. The exact sequence is proprietary.

Reactivity Notes

Mouse (80%), Rat (80%), Pig (88%), Bovine (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for TFF3 Antibody

Western Blot: TFF3 Antibody [NBP3-13358]

Western Blot: TFF3 Antibody [NBP3-13358]

Western Blot: TFF3 Antibody [NBP3-13358] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with TFF3 antibody (NBP3-13358) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358]

Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358]

Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358] - TFF3 antibody detects secreted TFF3 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. TFF3 stained by TFF3 antibody (NBP3-13358) diluted at 1:1500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358]

Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358]

Immunohistochemistry-Paraffin: TFF3 Antibody [NBP3-13358] - TFF3 antibody detects TFF3 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. TFF3 stained by TFF3 antibody (NBP3-13358) diluted at 1:1500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for TFF3 Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFF3

Trefoil Factor 3 is a member of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. Trefoil Factor 3 and two other related trefoil family member genes are found in a cluster on chromosome 21.

Long Name

Trefoil Factor 3

Alternate Names

ITF, TFI

Gene Symbol

TFF3

Additional TFF3 Products

Product Documents for TFF3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TFF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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