Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TFG Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TFG(NP_001007566) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TFG Antibody (OTI2C3) - Azide and BSA Free

Western Blot: TFG Antibody (OTI2C3)Azide and BSA Free [NBP2-74491]

Western Blot: TFG Antibody (OTI2C3)Azide and BSA Free [NBP2-74491]

Western Blot: TFG Antibody (OTI2C3) - Azide and BSA Free [NBP2-74491] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFG.
Immunohistochemistry: TFG Antibody (OTI2C3) - Azide and BSA Free [NBP2-74491]

Immunohistochemistry: TFG Antibody (OTI2C3) - Azide and BSA Free [NBP2-74491]

Immunohistochemistry: TFG Antibody (OTI2C3) - Azide and BSA Free [NBP2-74491] - Staining of paraffin-embedded Human prostate tissue using anti-TFG mouse monoclonal antibody.
Immunohistochemistry: TFG Antibody (OTI2C3) - Azide and BSA Free [NBP2-74491]

Immunohistochemistry: TFG Antibody (OTI2C3) - Azide and BSA Free [NBP2-74491]

Immunohistochemistry: TFG Antibody (OTI2C3) - Azide and BSA Free [NBP2-74491] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TFG mouse monoclonal antibody.
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Applications for TFG Antibody (OTI2C3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TFG

TRK-fused gene (TFG) has been identified as part of a chromosomal translocation [t(1,3)(q21;q11)] with NTRK1 in thyroid papillary carcinoma. TGF is implicated to play a role in modulating SHP-1 activity and has also been found to interact with TANK and NEMO, two proteins involved in the NF-kappaB signaling pathway.

Alternate Names

FLJ36137, protein TFG, TF6, TRK-fused gene, TRK-fused gene protein, TRKT3, TRKT3 oncogene

Gene Symbol

TFG

Additional TFG Products

Product Documents for TFG Antibody (OTI2C3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TFG Antibody (OTI2C3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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