TFIIB Antibody (PCRP-GTF2B-1D1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13934
![Flow Cytometry: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934]](https://resources.bio-techne.com/images/products/TFIIB-Antibody-PCRP-GTF2B-1D1-Flow-Cytometry-NBP3-13934-img0001.jpg "Flow Cytometry: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunoprecipitation, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PCRP-GTF2B-1D1
Concentration
0.2 mg/ml
Product Summary for TFIIB Antibody (PCRP-GTF2B-1D1)
Immunogen
Recombinant full-length human TFIIB protein (Uniprot: Q00403)
Localization
Nucleus, Nucleoplasm, nuclear bodies
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
34.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14055)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for TFIIB Antibody (PCRP-GTF2B-1D1)
Flow Cytometry: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934]
Flow Cytometry: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934] - Flow cytometric analysis of PFA-fixed HeLa cells. TFIIB antibody (PCRP-GTF2B-1D1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).![Protein Array: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934]](https://resources.bio-techne.com/images/products/TFIIB-Antibody-PCRP-GTF2B-1D1-Protein-Array-NBP3-13934-img0003.jpg "Protein Array: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934]")
Protein Array: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934]
Protein Array: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TFIIB Antibody (PCRP-GTF2B-1D1).Applications for TFIIB Antibody (PCRP-GTF2B-1D1)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunoprecipitation
1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TFIIB
Alternate Names
general transcription factor IIB, General transcription factor TFIIB, S300-II, TFIIBTF2BRNA polymerase II transcription factor IIB, transcription initiation factor IIB
Gene Symbol
GTF2B
UniProt
Additional TFIIB Products
Product Documents for TFIIB Antibody (PCRP-GTF2B-1D1)
Product Specific Notices for TFIIB Antibody (PCRP-GTF2B-1D1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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