TFIIB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32622

Novus Biologicals, part of Bio-Techne
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NBP1-32622
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Bovine (99%), Mouse (99%), Rat (99%), Rhesus Macaque (100%), Xenopus (96%), Zebrafish (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TFIIB Antibodies »

Product Summary for TFIIB Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human TFIIB. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (96%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TFIIB Antibody

Western Blot: TFIIB Antibody [NBP1-32622]

Western Blot: TFIIB Antibody [NBP1-32622]

Western Blot: TFIIB Antibody [NBP1-32622] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: TFIIB Antibody [NBP1-32622]

Immunocytochemistry/ Immunofluorescence: TFIIB Antibody [NBP1-32622]

Immunocytochemistry/Immunofluorescence: TFIIB Antibody [NBP1-32622] - TFIIB antibody detects TFIIB protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TFIIB stained by TFIIB antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10um.
Immunohistochemistry-Paraffin: TFIIB Antibody [NBP1-32622]

Immunohistochemistry-Paraffin: TFIIB Antibody [NBP1-32622]

Immunohistochemistry-Paraffin: TFIIB Antibody [NBP1-32622] - TFIIB antibody detects TFIIB protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TFIIB stained by TFIIB antibody diluted at 1:500.
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Applications for TFIIB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TFIIB

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.

Alternate Names

general transcription factor IIB, General transcription factor TFIIB, S300-II, TFIIBTF2BRNA polymerase II transcription factor IIB, transcription initiation factor IIB

Gene Symbol

GTF2B

Additional TFIIB Products

Product Documents for TFIIB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TFIIB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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