TFIIIC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14077

Novus Biologicals, part of Bio-Techne
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NBP2-14077-25ul
NBP2-14077
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TFIIIC Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: QVFIFLKKNAVIVDTTICDPHYNLARSSRPFERRLYVLNSMQDVENYWFDLQCVCLNTPLGVVRCPRVRKNSSTDQGSDEEGSLQKEQESAMDKHNLERKCAMLEYTTGSREVVDEGLIPGDGLGAAGLDSSFYG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFIIIC Antibody

Immunocytochemistry/ Immunofluorescence: TFIIIC Antibody [NBP2-14077]

Immunocytochemistry/ Immunofluorescence: TFIIIC Antibody [NBP2-14077]

Immunocytochemistry/Immunofluorescence: TFIIIC Antibody [NBP2-14077] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TFIIIC Antibody [NBP2-14077]

Immunohistochemistry-Paraffin: TFIIIC Antibody [NBP2-14077]

Immunohistochemistry-Paraffin: TFIIIC Antibody [NBP2-14077] - Staining of human salivary gland shows strong nuclear and cytoplasmic positivity in glandular cells.

Applications for TFIIIC Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF,Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFIIIC

General transcription factor 3C polypeptide 1 (GTF3C1/TFIIIC220) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. The functional contribution of GTF3C1/TFIIIC220 to the TFIIIC complex has not been fully determined.

Alternate Names

general transcription factor 3C polypeptide 1, general transcription factor IIIC, polypeptide 1 (alpha subunit), general transcription factor IIIC, polypeptide 1 (alpha subunit, 220kD ), general transcription factor IIIC, polypeptide 1, alpha 220kDa, TF3C-alpha, TFIIIC, TFIIIC 220 kDa subunit, TFIIIC box B-binding subunit, TFIIIC220DKFZp686A111, TFIIICalpha, Transcription factor IIIC 220 kDa subunit, Transcription factor IIIC subunit alpha

Gene Symbol

GTF3C1

Additional TFIIIC Products

Product Documents for TFIIIC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TFIIIC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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