TFIISH Antibody (2C9)

Novus Biologicals, part of Bio-Techne | Catalog # H00006920-M06

Novus Biologicals, part of Bio-Techne
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H00006920-M06
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2C9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TFIISH Antibodies »

Product Specifications

Immunogen

TCEA3 (AAH41613, 1 a.a. ~ 348 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGQEEELLRIAKKLEKMVARKNTEGALDLLKKLHSCQMSIQLLQTTRIGVAVNGVRKHCSDKEVVSLAKVLIKNWKRLLDSPGPPKGEKGEEREKAKKKEKGLECSDWKPEAGLSPPRKKREDPKTRRDSVDSKSSASSSPKRPSVERSNSSKSKAESPKTPSSPLTPTFASSMCLLAPCYLTGDSVRDKCVEMLSAALKADDDYKDYGVNCDKMASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISAGLIAKMTAEEMASDELRELRNAMTQEAIREHQMAKTGGTTTDLFQCSKCKKKNCTYNQVQTRSADEPMTTFVLCNECGNRWKFC

Localization

Nuclear

Specificity

TCEA3 - transcription elongation factor A (SII), 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TFIISH Antibody (2C9)

Western Blot: TFIISH Antibody (2C9) [H00006920-M06]

Western Blot: TFIISH Antibody (2C9) [H00006920-M06]

Western Blot: TFIISH Antibody (2C9) [H00006920-M06] - Analysis of TCEA3 expression in transfected 293T cell line by TCEA3 monoclonal antibody (M06), clone 2C9. Lane 1: TCEA3 transfected lysatE (39 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: TFIISH Antibody (2C9) [H00006920-M06]

Immunocytochemistry/ Immunofluorescence: TFIISH Antibody (2C9) [H00006920-M06]

Immunocytochemistry/Immunofluorescence: TFIISH Antibody (2C9) [H00006920-M06] - Analysis of monoclonal antibody to TCEA3 on HeLa cell. Antibody concentration 10 ug/ml
Immunoprecipitation: TFIISH Antibody (2C9) [H00006920-M06]

Immunoprecipitation: TFIISH Antibody (2C9) [H00006920-M06]

Immunoprecipitation: TFIISH Antibody (2C9) [H00006920-M06] - Analysis of TCEA3 transfected lysate using anti-TCEA3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TCEA3 rabbit polyclonal antibody.

Applications for TFIISH Antibody (2C9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TFIISH

TCEA3 is a trancription elongation factor. It is necessary for efficient RNA polymerase II transcription elongation past template encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Alternate Names

rhabdomyosarcoma antigen MU-RMS-40.22, TFIIS, TFIIS.H, TFIISH, transcription elongation factor A (SII), 3, transcription elongation factor A protein 3, Transcription elongation factor S-II protein 3, Transcription elongation factor TFIIS.h

Entrez Gene IDs

6920 (Human)

Gene Symbol

TCEA3

OMIM

604128 (Human)

UniProt

AAH41613 (Human)

Additional TFIISH Products

Product Documents for TFIISH Antibody (2C9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TFIISH Antibody (2C9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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