TfR (Transferrin R) Antibody (040)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89744
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (040) [NBP2-89744]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-040-Immunocytochemistry-Immunofluorescence-NBP2-89744-img0001.jpg "Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (040) [NBP2-89744]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 040
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for TfR (Transferrin R) Antibody (040)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human TfR (Transferrin R) (Accession#: AAA61153.1; Cys89-Phe760).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for TfR (Transferrin R) Antibody (040)
Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (040) [NBP2-89744]
Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (040) [NBP2-89744] - Staining of Human TFRC in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TFRC monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.![Flow Cytometry: TfR (Transferrin R) Antibody (040) [NBP2-89744]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-040-Flow-Cytometry-NBP2-89744-img0002.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (040) [NBP2-89744]")
Flow Cytometry: TfR (Transferrin R) Antibody (040) [NBP2-89744]
Flow Cytometry: TfR (Transferrin R) Antibody (040) [NBP2-89744] - Analysis of Human TFRC(CD71) expression on U937 cells. Cells were stained with purified anti-Human TFRC(CD71), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.Applications for TfR (Transferrin R) Antibody (040)
Application
Recommended Usage
Flow Cytometry
1:25-1:100
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TfR (Transferrin R)
Long Name
Transferrin Receptor
Alternate Names
CD71, TfR (TransferrinR), TFR1, TFRC, TRFR
Gene Symbol
TFRC
Additional TfR (Transferrin R) Products
Product Documents for TfR (Transferrin R) Antibody (040)
Product Specific Notices for TfR (Transferrin R) Antibody (040)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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