TfR (Transferrin R) Antibody (OX26) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB200-585

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OX26

Format

BSA Free

Concentration

1.0 mg/ml

View all TfR (Transferrin R) Antibodies »

Product Specifications

Immunogen

PHA activated rat lymphocytes.

Specificity

Labels dividing cells of various types but also binds to a number of non-dividing normal tissues and endothelium of brain capillaries.

Marker

Recycling Endosome Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TfR (Transferrin R) Antibody (OX26) - BSA Free

Western Blot: TfR (Transferrin R) Antibody (OX26)BSA Free [NB200-585]

Western Blot: TfR (Transferrin R) Antibody (OX26)BSA Free [NB200-585]

Western Blot: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Total protein from rat adrenal gland cells (PC12) and rat placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD71 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (OX26) - BSA Free [NB200-585]

Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (OX26) - BSA Free [NB200-585]

Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (OX26) [NB200-585] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti TfR/Transferrin R (OX26) NB200-585 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (red) at a 1:200 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (OX26) - BSA Free [NB200-585]

Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (OX26) - BSA Free [NB200-585]

Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Analysis of FFPE mouse spleen using TfR (OX26) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Plasma membrane staining was observed. Staining was performed by Histowiz.
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Applications for TfR (Transferrin R) Antibody (OX26) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TfR (Transferrin R)

OX26 TfR clone is useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. In comes in a variety of pre-conjugated fluorophores for histochemical and flow applications.

Long Name

Transferrin Receptor

Alternate Names

CD71, TfR (TransferrinR), TFR1, TFRC, TRFR, OX26 monoclonal antibody, OX26 transferrin receptor

Entrez Gene IDs

64678 (Rat)

Gene Symbol

TFRC

UniProt

Q99376 (Rat)

Additional TfR (Transferrin R) Products

Product Documents for TfR (Transferrin R) Antibody (OX26) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TfR (Transferrin R) Antibody (OX26) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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