Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

CyTOF-ready, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MF9

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all TGF-alpha Antibodies »

Product Specifications

Immunogen

A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF-alpha (exact sequence is proprietary) (Uniprot: P01135)

Localization

Cytoplasmic and Extracellular (secreted)

Specificity

This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34296).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for TGF-alpha Antibody (MF9) - Azide and BSA Free

Immunohistochemistry-Paraffin: TGF-alpha Antibody (MF9) - Azide and BSA Free [NBP2-34683]

Immunohistochemistry-Paraffin: TGF-alpha Antibody (MF9) - Azide and BSA Free [NBP2-34683]

Immunohistochemistry-Paraffin: TGF-alpha Antibody (MF9) - Azide and BSA Free [NBP2-34683] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma Stained with TGF alpha Monoclonal Antibody (MF9).

Applications for TGF-alpha Antibody (MF9) - Azide and BSA Free

Application
Recommended Usage

ELISA

1-5ug/ml for coating

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Western Blot

0.5-1.0ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: TGF-alpha

Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Long Name

Transforming Growth Factor alpha

Alternate Names

TGFA, TGFalpha

Gene Symbol

TGFA

Additional TGF-alpha Products

Product Documents for TGF-alpha Antibody (MF9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TGF-alpha Antibody (MF9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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