TGF-alpha Antibody (SPM357) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08270B

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM357

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TGF-alpha Antibodies »

Product Specifications

Immunogen

A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF-alpha (Uniprot: P01135)

Localization

Cytoplasmic and Extracellular (secreted)

Specificity

This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for TGF-alpha Antibody (SPM357) [Biotin]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TGF-alpha

Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Long Name

Transforming Growth Factor alpha

Alternate Names

TGFA, TGFalpha

Gene Symbol

TGFA

Additional TGF-alpha Products

Product Documents for TGF-alpha Antibody (SPM357) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TGF-alpha Antibody (SPM357) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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