TGF-beta 1 Antibody (OTI1E12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46108

Novus Biologicals, part of Bio-Techne
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NBP2-46108
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Conjugate
Catalog #
Alexa Fluor 350
NBP2-74495AF350
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Alexa Fluor 405
NBP2-74495AF405
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Alexa Fluor 488
NBP2-74495AF488
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Alexa Fluor 532
NBP2-74495AF532
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Alexa Fluor 594
NBP2-74495AF594
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Alexa Fluor 647
NBP2-74495AF647
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Alexa Fluor 700
NBP2-74495AF700
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Alexa Fluor 750
NBP2-74495AF750
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Allophycocyanin
NBP2-74495APC
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Allophycocyanin/Cy7
NBP2-74495APCCY7
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Biotin
NBP2-74495B
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CoraFluor 1
NBP2-74495CL1
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DyLight 350
NBP2-74495UV
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DyLight 405
NBP2-74495V
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DyLight 488
NBP2-74495G
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DyLight 550
NBP2-74495R
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DyLight 594
NBP2-74495DL594
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DyLight 650
NBP2-74495C
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DyLight 680
NBP2-74495FR
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DyLight 755
NBP2-74495IR
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FITC
NBP2-74495F
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HRP
NBP2-74495H
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Janelia Fluor 525
NBP2-74495JF525
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Janelia Fluor 549
NBP2-74495JF549
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Janelia Fluor 585
NBP2-74495JF585
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Janelia Fluor 635
NBP2-74495JF635
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Janelia Fluor 646
NBP2-74495JF646
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Janelia Fluor 669
NBP2-74495JF669
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mFluor Violet 450 SE
NBP2-74495MFV450
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mFluor Violet 500 SE
NBP2-74495MFV500
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mFluor Violet 610 SE
NBP2-74495MFV610
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PE
NBP2-74495PE
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PE/Atto594
NBP2-74495PEATT594
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PE/Cy5.5
NBP2-74495PECY55
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PE/Cy7
NBP2-74495PECY7
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PerCP
NBP2-74495PCP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1E12

Concentration

1 mg/ml

View all TGF-beta 1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TGFB1(NP_000651) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

28.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TGF-beta 1 Antibody (OTI1E12)

Western Blot: TGF-beta 1 Antibody (OTI1E12) [NBP2-46108]

Western Blot: TGF-beta 1 Antibody (OTI1E12) [NBP2-46108]

Western Blot: TGF-beta 1 Antibody (OTI1E12) [NBP2-46108] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGF-beta 1.
Flow Cytometry: TGF-beta 1 Antibody (OTI1E12) [NBP2-46108]

Flow Cytometry: TGF-beta 1 Antibody (OTI1E12) [NBP2-46108]

Flow Cytometry: TGF-beta 1 Antibody (OTI1E12) [NBP2-46108] - Analysis of SF295 cells, using TGFB1 antibody,(Red), compared to a nonspecific negative control antibody (Blue).
Immunofluorescence: TGF-beta 1 Antibody (OTI1E12) [NBP2-46108]

Immunofluorescence: TGF-beta 1 Antibody (OTI1E12) [NBP2-46108]

Immunofluorescence: TGF-beta 1 Antibody (OTI1E12) [NBP2-46108] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY TGF-Beta 1.
View 1 more images

Applications for TGF-beta 1 Antibody (OTI1E12)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TGF-beta 1

TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, which includes three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25kDa homodimer composed of two 12.5kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule - the amino acid sequence between human and mouse differ only by one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues such as placenta and kidneys.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Gene Symbol

TGFB1

Additional TGF-beta 1 Products

Product Documents for TGF-beta 1 Antibody (OTI1E12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TGF-beta 1 Antibody (OTI1E12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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