TGF-beta 1 Antibody (PD00-17)

Western Blot: TGF-beta 1 Antibody (PD00-17) [NBP3-33008]

Western Blot: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Western blot analysis of TGF-beta 1 on different lysates with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/2,000 dilution.

Lane 1: Raji cell lysate (15 ug/Lane)
Lane 2: K-562 cell lysate (15 ug/Lane)
Lane 3: Mouse spleen tissue lysate (20 ug/Lane)
Lane 4: Rat spleen tissue lysate (20 ug/Lane)

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 3 minutes 54 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33008) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TGF-beta 1 Antibody (PD00-17) [NBP3-33008]

Immunohistochemistry: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33008) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.