TGF-beta 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-03276

Novus Biologicals, part of Bio-Techne
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NBP1-03276
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Equine

Cited:

Mouse, Equine

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TGF-beta 1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide from human TGF-beta 1.

Reactivity Notes

Mouse reactivity reported in (PMID: 27713548), Equine reactivity reported in (PMID: 23611525).

Specificity

A BLAST analysis was used to suggest reactivity with this protein from most mammalian sources based on 100% homology for the immunogen sequence. Cross-reactivity is expected with TGF-beta 1 from non-mammalian sources as only a single amino acid residue change is found within the immunogen sequence from many other organisms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TGF-beta 1 Antibody

Western Blot: TGF-beta 1 Antibody [NBP1-03276] -

Western Blot: TGF-beta 1 Antibody [NBP1-03276] - Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). The blot was incubated with a 1:200 dilution of the antibody at room temperature for 1 h followed by detection using labeled Goat-a-Rabbit IgG (H&L).
Immunohistochemistry-Paraffin: TGF-beta 1 Antibody [NBP1-03276]

Immunohistochemistry-Paraffin: TGF-beta 1 Antibody [NBP1-03276]

Immunohistochemistry-Paraffin: TGF-beta 1 Antibody [NBP1-03276] - Staining of human spleen tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Applications for TGF-beta 1 Antibody

Application
Recommended Usage

ELISA

1:10000 - 1:20000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1:100 - 1:500
Application Notes
IHC-Frozen reactivity reported in (PMID: 23611525). .

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: TGF-beta 1

TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, which includes three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25kDa homodimer composed of two 12.5kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule - the amino acid sequence between human and mouse differ only by one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues such as placenta and kidneys.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Entrez Gene IDs

7040 (Human)

Gene Symbol

TGFB1

UniProt

P01137 (Human)

Additional TGF-beta 1 Products

Product Documents for TGF-beta 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TGF-beta 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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