TGF-beta 1/1.2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AB-101-NA

R&D Systems, part of Bio-Techne
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AB-101-NA
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Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse

Applications

Validated:

Neutralization, Western Blot

Cited:

Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

View all TGF-beta 1/1.2 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta1 (R&D Systems, Catalog # 240-B)
Ala279-Ser390
Accession # P01137

Specificity

Detects TGF‑ beta1/1.2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant amphibian TGF‑ beta5 is observed and less than 2% cross-reactivity with recombinant human TGF‑ beta3, recombinant chicken TGF‑ beta3, and porcine TGF‑ beta2 is observed.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for TGF-beta 1/1.2 Antibody

TGF‑ beta1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta1/1.2 Antibody.

TGF‑ beta1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta1/1.2 Antibody.

Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Chicken Anti-TGF-beta 1/1.2 Polyclonal Antibody (Catalog # AB-101-NA). The ND50 is typically 0.2-0.6 µg/mL.

Applications for TGF-beta 1/1.2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human TGF-beta 1 (Catalog # 240-B)
Recombinant Human TGF-beta 1.2 (Catalog # 304-B3)

Neutralization

Measured by its ability to neutralize TGF‑ beta1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line [Tsang, M. et al. (1995) Cytokine 7:389]. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 0.25 ng/mL Recombinant Human TGF‑ beta1 and 7.5 ng/mL Recombinant Mouse IL‑4.

Formulation, Preparation, and Storage

Purification

Ion exchange-purified from egg yolks

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 1/1.2

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts.

Long Name

Transforming Growth Factor beta 1 and 1.2

Alternate Names

TGFb1.2

Entrez Gene IDs

7040 (Human); 21803 (Mouse); 59086 (Rat)

Gene Symbol

TGFB1

UniProt

P01137 (Human)

Additional TGF-beta 1/1.2 Products

Product Documents for TGF-beta 1/1.2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TGF-beta 1/1.2 Antibody

For research use only

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