THAP11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49463

Novus Biologicals, part of Bio-Techne
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NBP1-49463
NBP1-49463SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (95%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all THAP11 Antibodies »

Product Specifications

Immunogen

Partial recombinant human THAP11 protein expressed in E. coli [Swiss-Prot# Q96EK4]

Reactivity Notes

Immunogen sequence has 94% identity to rat and 95% identity to bovine.

Localization

Nucleus. Cytoplasm Note: May be regulated by shuttling of the protein between the cytoplasm and nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for THAP11 Antibody

Western Blot: THAP11 Antibody [NBP1-49463]

Western Blot: THAP11 Antibody [NBP1-49463]

Western Blot: THAP11 Antibody [NBP1-49463] - Analysis of THAP11 in HeLa whole cell extracts.
Immunocytochemistry/ Immunofluorescence: THAP11 Antibody [NBP1-49463]

Immunocytochemistry/ Immunofluorescence: THAP11 Antibody [NBP1-49463]

Immunocytochemistry/Immunofluorescence: THAP11 Antibody [NBP1-49463] - Immunocytochemical analysis of THAP11 in HeLa cells.
Immunohistochemistry: THAP11 Antibody [NBP1-49463]

Immunohistochemistry: THAP11 Antibody [NBP1-49463]

Immunohistochemistry: THAP11 Antibody [NBP1-49463] - Analysis of THAP11 in mouse skin
View 1 more images

Applications for THAP11 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:300

Immunohistochemistry-Paraffin

1:300

Western Blot

1:1000
Application Notes
This THAP11 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~26 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: THAP11

Thanatos-associated protein (THAP) domain is an evolutionarily conserved C2-CH (C-X2-4-C-X35-50-C-X2-H) zinc finger motif which has sequence-specific DNA-binding activity and twelve THAP proteins, each containing an N-terminally located THAP domain, have been identified in humans (THAP0 to THAP11), some of which (THAP0, -1, -2, -4, -7, and -11) are also conserved in mice/rats. Individual THAP proteins have been implicated in a diverse array of physiological processes, including cell proliferation, regulation of transcription, apoptosis, and maintenance of embryonic stem (ES) cell pluripotency. THAP11 is regulated by shuttling between cytoplasm - nucleus, and is considered a transcriptional repressor that plays a central role in embryogenesis as well as in ES cell pluripotency. It act as a sequence-specific DNA-binding factor which represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers. THAP11 has been suggested as a candidate for polyglutamine disorders based on polymorphism and protein-folding simulation studies. RONIN is the mouse homolog of THAP11 and its homozygous deletion happened to be embryonically lethal in mice wherein was shown to be essential factor underlying embryogenesis/ES cell pluripotency via its activational/ repressional effects on transcription. THAP11 also functions as a negative regulator of cell growth in human HepG2 hepatoma cells through transcriptional repression of the proto-oncogene MYC.

Long Name

THAP Domain Containing 11

Alternate Names

CTG-B43a, CTG-B45d, Ronin

Entrez Gene IDs

57215 (Human)

Gene Symbol

THAP11

UniProt

Q96EK4

Additional THAP11 Products

Product Documents for THAP11 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for THAP11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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