Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all THEM4 Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid synthetic peptide near the center of human THEM4. The immunogen is located within amino acids 60 - 110 of THEM4. Amino Acid Squence: EGDSWKRLPSYKRTPTE

Specificity

The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for THEM4 Antibody - BSA Free

Western Blot: THEM4 AntibodyBSA Free [NBP1-77360]

Western Blot: THEM4 AntibodyBSA Free [NBP1-77360]

Western Blot: THEM4 Antibody [NBP1-77360] - Human liver tissue lysate with THEM4 antibody at (A) 1 and (B) 2 ug/ml.
THEM4 Antibody - BSA Free

Immunohistochemistry: THEM4 Antibody - BSA Free [NBP1-77360] -

Immunohistochemistry: THEM4 Antibody - BSA Free [NBP1-77360] - Immunohistochemistry of THEM4 in human liver tissue with THEM4 antibody at 2.5 ug/mL.
THEM4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: THEM4 Antibody - BSA Free [NBP1-77360] -

Immunocytochemistry/ Immunofluorescence: THEM4 Antibody - BSA Free [NBP1-77360] - Immunofluorescence of THEM4 in human liver tissue with THEM4 antibody at 20 ug/mL.

Applications for THEM4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: THEM4

THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program. The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.

Alternate Names

C-terminal modulator protein, CTMPCarboxyl-terminal modulator protein, MGC29636, thioesterase superfamily member 4

Gene Symbol

THEM4

UniProt

NP_444283 (Human)

Additional THEM4 Products

Product Documents for THEM4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for THEM4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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