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Themis Antibody (SR2184)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22411

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-22411-100ul
NBP3-22411-50ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2184

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from Themis (Uniprot #: Q8N1K5)

Specificity

Detects endogenous levels of total Themis

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Themis Antibody (SR2184)

Western Blot: Themis Antibody (SR2184) [NBP3-22411] -

Western Blot: Themis Antibody (SR2184) [NBP3-22411] - Western blot analysis using NBP3-22411 in Jurkat cell lysate.

Applications for Themis Antibody (SR2184)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Themis

THEMIS (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling.

Long Name

Thymocyte-expressed Molecule Involved in Selection

Alternate Names

C6orf190, C6orf207, Gasp, SPOT, TSEPA

Gene Symbol

THEMIS

Additional Themis Products

Product Documents for Themis Antibody (SR2184)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Themis Antibody (SR2184)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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