Thioredoxin-1 Antibody (3H8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26266

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3H8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Thioredoxin-1 Antibodies »

Product Summary for Thioredoxin-1 Antibody (3H8)

Immunogen

A synthesized peptide derived from Human Thioredoxin-1 [UniProt P10599]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Thioredoxin-1 Antibody (3H8)

Thioredoxin-1 Antibody (3H8)

Western Blot: Thioredoxin-1 Antibody (3H8) [NBP3-26266] -

Western Blot: Thioredoxin-1 Antibody (3H8) [NBP3-26266] - Positive Western Blot detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate.
All lanes: Thioredoxin-1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 10, 12 kDa
Observed band size: 12 kDa

Applications for Thioredoxin-1 Antibody (3H8)

Application
Recommended Usage

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Thioredoxin-1

Thioredoxins (Trxs) are a group of small ubiquitous proteins that are key regulators of cellular redox balance. The mammalian Trx family includes the secreted and cellular protein (Trx-1), the mitochondria-specific Trx-2, the Trx-like cytosolic protein p32TrxL, a truncated version of Trx-1 known as Trx80, and the thioredoxin-related protein, TRP-14. Trx-1 is secreted by lymphocytes, hepatocytes, fibroblasts, and several tumor cells. Trx-1 acts as a growth factor an, antioxidant, a cofactor that provides reducing equivalents, and a transcriptional regulator. Expression of Trx-1 is increased under various stress conditions such as hypoxia and viral and bacterial infections. It also serves as a biomarker for rheumatoid arthritis.

Alternate Names

Thioredoxin1, Trx1, TXN, TXN1

Gene Symbol

TXN

Additional Thioredoxin-1 Products

Product Documents for Thioredoxin-1 Antibody (3H8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Thioredoxin-1 Antibody (3H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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