Thioredoxin-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48873

Novus Biologicals, part of Bio-Techne
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NBP2-48873
NBP2-48873-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Thioredoxin-1 Antibodies »

Product Summary for Thioredoxin-1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SLSEKYSNVIFLEVDVDDCQDVASECEVKCMPTFQFFKKGQKVGEFSGA

Reactivity Notes

Mouse (88%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Thioredoxin-1 Antibody

Western Blot: Thioredoxin-1 Antibody [NBP2-48873]

Western Blot: Thioredoxin-1 Antibody [NBP2-48873]

Western Blot: Thioredoxin Antibody [NBP2-48873] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Lane 4: Human plasma
Lane 5: Human Liver tissue
Lane 6: Human Tonsil tissue
Immunocytochemistry/ Immunofluorescence: Thioredoxin-1 Antibody [NBP2-48873]

Immunocytochemistry/ Immunofluorescence: Thioredoxin-1 Antibody [NBP2-48873]

Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-48873] - Staining of human cell line U-251 MG shows localization to nucleus and cytosol. Antibody staining is shown in greeen.
Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody [NBP2-48873]

Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody [NBP2-48873]

Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-48873] - Staining of human kidney.
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Applications for Thioredoxin-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thioredoxin-1

Thioredoxins (Trxs) are a group of small ubiquitous proteins that are key regulators of cellular redox balance. The mammalian Trx family includes the secreted and cellular protein (Trx-1), the mitochondria-specific Trx-2, the Trx-like cytosolic protein p32TrxL, a truncated version of Trx-1 known as Trx80, and the thioredoxin-related protein, TRP-14. Trx-1 is secreted by lymphocytes, hepatocytes, fibroblasts, and several tumor cells. Trx-1 acts as a growth factor an, antioxidant, a cofactor that provides reducing equivalents, and a transcriptional regulator. Expression of Trx-1 is increased under various stress conditions such as hypoxia and viral and bacterial infections. It also serves as a biomarker for rheumatoid arthritis.

Alternate Names

Thioredoxin1, Trx1, TXN, TXN1

Gene Symbol

TXN

Additional Thioredoxin-1 Products

Product Documents for Thioredoxin-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Thioredoxin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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