Thrombospondin-1 Antibody (A4.1)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-554
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Chicken
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # A4.1
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Calcium-replete, native purified human TSP from the supernatant of thrombin-activated platelets
Epitope
N-terminal half of the central stalk-like region of TSP 1 which is recovered as a 50 kDa fragment after chymotryptic digestion of TSP.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Cell Membrane
Specificity
It shows no cross-reaction with fibronectin, fibrinogen or Von Willebrand factor.
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for Thrombospondin-1 Antibody (A4.1)
![Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554]](https://resources.bio-techne.com/images/products/Thrombospondin-1-Antibody-A4-1-Western-Blot-NB600-554-img0003.jpg "Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554]")
Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554]
Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554] - Analysis of HUVEC Cells.![Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554]](https://resources.bio-techne.com/images/products/Thrombospondin-1-Antibody-A4-1-Western-Blot-NB600-554-img0002.jpg "Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554]")
Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554]
Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554] - Analysis of Human Cells in a Non-reducing Gel.Applications for Thrombospondin-1 Antibody (A4.1)
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Immunofluorescence
1:10 - 1:500
Western Blot
1 - 2 ug/ml
Application Notes
May be useful in Flow, IF, and WB. WB: Detects an approx. 450 kDa (nonreduced), which corresponds to the predicted molecular weight of Thrombospondin. This antibody blocks anti-angiogenic activity of TSP 1 by inhibiting its binding to TSP Receptor / CD36. This antibody inhibits the adhesion of human melanoma G361 cells, keratinocytes, squamous carcinoma cells, and rat smooth muscle cells to TSP 1. It does not inhibit aggregation of thrombin-induced platelets.
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Thrombospondin-1
Alternate Names
THBS1, Thrombospondin1, TSP-1
Gene Symbol
THBS1
Additional Thrombospondin-1 Products
Product Documents for Thrombospondin-1 Antibody (A4.1)
Product Specific Notices for Thrombospondin-1 Antibody (A4.1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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