Thrombospondin-1 Antibody (A6.1)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2059
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat
Applications
Validated:
Block/Neutralize, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Block/Neutralize, IF/IHC, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, PCR, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # A6.1
Concentration
0.2 mg/ml
Product Specifications
Immunogen
BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
Localization
Secretory granules, Golgi complex, endoplasmic reticulum
Specificity
This is specific to 450 kD protein, a non-reduced form, and 170 to 180 kD protein, a reduced form, of Thrombospondin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Thrombospondin-1 Antibody (A6.1)
Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059]
Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Representative photomicrographs showing Thrombospondin-1 (A) immunostaining in duodenal sections of 1 non-CD controls (NC) and 1 active celiac disease (ACD) patient. Isotype control staining is also shown. Right panels: positive cells were counted in at least 5 fields/section of duodenal samples of 5 NC and 5 ACD patients and expressed as mean +/- SD of all experiments. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0100980), licensed under a CC-BY license.Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]
Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Staining in human placenta.Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]
Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Ang II increases the number of TSP-1 positive cells in mouse heart and skin.IHC staining of TSP-1 was performed on paraffin sections from the skin of PBS and Ang II treated WT mice. Representative photographs are shown from five animals per group. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0109763), licensed under a CC-BY license.Applications for Thrombospondin-1 Antibody (A6.1)
Application
Recommended Usage
Immunohistochemistry
1:25-1:50
Immunohistochemistry-Paraffin
1:25-1:50
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT. Use in Blocking/Neutralizing reported in scientific literature (PMID: 19509293). Use in Western blot reported in reported in scientific literature (PMID: 27447109). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24971453).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.4), 0.2% BSA, Tween-20
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Thrombospondin-1
Alternate Names
THBS1, Thrombospondin1, TSP-1
Gene Symbol
THBS1
UniProt
Additional Thrombospondin-1 Products
Product Documents for Thrombospondin-1 Antibody (A6.1)
Product Specific Notices for Thrombospondin-1 Antibody (A6.1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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