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Key Product Details

Species Reactivity

Human, Bovine

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # A65M

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Extracellular matrix material from cultured bovine corneal endothelial cells

Epitope

Epitope is only present in thrombospondin prepared in low-calcium (0.1 mmol/L) buffers.

Specificity

Highly specific for the low-calcium (0.1 mmol/L) conformation of human TSP1. There is no evidence of cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Thrombospondin-1 Antibody (A65M) - BSA Free

Application
Recommended Usage

ELISA

1:200

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500
Application Notes
IHC: NBP1-05185 for immunostaining of frozen periodate-lysine-paraformaldehyde-fixed sections of bovine and human tissues. NBP1-05185 can also be used to probe for the low-calcium conformation of thrombospondin. (1)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Thrombospondin-1

Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Alternate Names

THBS1, Thrombospondin1, TSP-1

Gene Symbol

THBS1

Additional Thrombospondin-1 Products

Product Documents for Thrombospondin-1 Antibody (A65M) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Thrombospondin-1 Antibody (A65M) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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