Thrombospondin-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52410

Novus Biologicals, part of Bio-Techne
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NBP1-52410
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Key Product Details

Species Reactivity

Human, Mouse, Porcine, Bovine, Equine, Monkey

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Thrombospondin-1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence NRIPESGGDNSVFD-C, from the N Terminus of the Thrombospondin-1 protein sequence according to NP_003237.2.

Epitope

N-Terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Thrombospondin-1 Antibody

Western Blot: Thrombospondin-1 Antibody [NBP1-52410]

Western Blot: Thrombospondin-1 Antibody [NBP1-52410]

Western Blot: Thrombospondin-1 Antibody [NBP1-52410] - (1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Thrombospondin-1 Antibody [NBP1-52410]

Immunocytochemistry/ Immunofluorescence: Thrombospondin-1 Antibody [NBP1-52410]

Immunocytochemistry/Immunofluorescence: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/Plasma Membrane staining.
Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP1-52410]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP1-52410]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of anti-THBS1 / Thrombospondin antibody with human placenta at concentration 3.75 ug/ml.
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Applications for Thrombospondin-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

1:128000

Western Blot

0.3-1 ug/ml
Application Notes
IHC: NBP1-52410 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Thrombospondin-1 antibody validated for FLOW from a verified customer review..
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-52410 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

TBS (pH 7.3) and 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Thrombospondin-1

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis.

Alternate Names

THBS1, Thrombospondin1, TSP-1

Entrez Gene IDs

7057 (Human)

Gene Symbol

THBS1

UniProt

P07996

Additional Thrombospondin-1 Products

Product Documents for Thrombospondin-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Thrombospondin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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